Concentration of mitochondrial DNA mutations by cytoplasmic transfer from platelets to cultured mouse cells
نویسندگان
چکیده
منابع مشابه
Mitochondrial DNA Mutations, Pathogenicity and Inheritance
Mitochondria contain their own DNA (mtDNA), which codes for 13 proteins (all subunits of the respiratory chain complexes), 22 tRNAs and 2 rRNAs. Several mtDNA point mutations as well as deletions have been shown to be causative in well-defined mitochondrial disorders. A mixture of mutated and wild type mtDNA (heteroplasmy) is found in most of these disorders. Inheritance of mtDNA is maternal, a...
متن کاملMethods for Efficient Elimination of Mitochondrial DNA from Cultured Cells
Here, we document that persistent mitochondria DNA (mtDNA) damage due to mitochondrial overexpression of the Y147A mutant uracil-N-glycosylase as well as mitochondrial overexpression of bacterial Exonuclease III or Herpes Simplex Virus protein UL12.5M185 can induce a complete loss of mtDNA (ρ0 phenotype) without compromising the viability of cells cultured in media supplemented with uridine and...
متن کاملThe Effect of FBS Concentration on Cryopreservation of Isolated Spermatogonial Cells from Neonatal Mouse by Macs Method
Purpose: The purpose of this study was the separation of spermatogonial cells from prepubertal NMRI mouse testis by magnetic activating cell sorting (MACS) method and assessment of FBS concentration on surviving these cells after cryopreservation. Materials and Methods: Testes from 6-days-old mouse removed and digested in two enzymatic mediums. First 8-9 min at medium containing collagenase, tr...
متن کاملCytoplasmic transfer of DNA containing simian virus 40 sequences into mouse 3T3 cells.
This paper describes the rare cytoplasmic transmission of defective simian virus 40 (SV40) viral DNA from enucleated cells (i.e., cytoplasts) of the SV40-transformed mouse cell line SVT2 (chloramphenicol-resistant) into cybrid cells formed by fusion of these cytoplasts with BALB/c 3T3 cells (thymidine kinase-deficient). The cybrids were selected in medium containing 1% serum, bromodeoxyuridine,...
متن کاملHigh Concentration of Cytoplasmic Metallothionein Increased Resistance to Chlorambucil in Cultured Cells with a Updated Version
Cultured cells with elevated levels of cytoplasmic metalloidionein (MT), a small-molecular-weight protein extremely rich in cysteine. showed resistance against the alkylating drug chlorambucil. One human epithelial line (HE) and one mouse fibroblast line (CI 1D) were used, as well as substrains of both cell lines (HE10oand CI 1D10o).The substrains contain large amounts of MT. Dose-survival curv...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2019
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0213283